ProAxsis NEATstik® (Neutrophil Elastase Airways Test)
$0.00
NEATstik® is a single use lateral flow format test for the qualitative detection of active neutrophil elastase (NE) in sputum samples. This product is for research use only. It is not for use in diagnostic procedures.
Neutrophil elastase (NE) is a biomarker of infection and inflammation. NE has long been used as an endpoint in clinical studies aimed at evaluating the efficacy of new anti-elastase inhibitors or other anti-inflammatory therapies
Read more about NE as a neutrophil serine proteases (NSP) and biomarker.
Measurement Principle
NEATstik® utilises ProteaseTag® technology to specifically detect active NE in sputum samples.
NEATstik® consists of a lateral flow test strip enclosed within a plastic cassette. The test strip comprises of a membrane and pads on a solid support with applied CONJUGATE and two reaction lines: a TEST (T) line and a CONTROL (C) line. The conjugate is a mixture of a ProteaseTag® (designed to specifically interact with active NE) and colored gold particles. The TEST (T) line consists of an antibody which can detect NE and the CONTROL (C) line can detect the conjugate.
Following dilution and gentle mixing of the sputum sample, a small volume is added to the sample port on NEATstik® to begin the testing process. The diluted sample travels along the test strip and interacts with the conjugate. The ProteaseTag® in the conjugate will bind to active NE present in the sample, travel up the test strip and bind to the TEST (T) line. Excess conjugate will bind to the CONTROL (C) line.
The appearance of the TEST line confirms the presence of active NE in the sample. The appearance of the CONTROL line confirms that the test has been performed correctly.
Each multipack box contains 10 individual kits.
| 1x | NEATstik® lateral flow test in a sealed foil pouch |
| 1x | NEATstik® sample dilution buffer (20 mL) |
| 1x | Sputum dilution pot |
| 1x | Dual bulb pipette |
| 1x | Graduated pipette |
| 1x | Instruction leaflet |
| Format | Lateral flow |
| Species | Human |
| Sample | Diluted sputum samples only |
| If the TEST (T) line is visible | Confirms the presence of active NE in the sputum sample |
| If the TEST (T) line is not visible | Active NE is not detectable in the sputum sample |
| Specificity | Only detects active NE |
| Run Time | 10 minute incubation |
To measure the concentration of active NE in a sputum sample, use of ProteaseTag®Active Neutrophil Elastase Immunoassay is recommended (available from DiaPharma).
Active Neutrophil Elastase and Active Proteinase 3 are serine proteases and are currently being used as endpoint measurements in researching efficacy of new inhibitors to these proteases or other anti-inflammatory therapies. With infection or inflammation in respiratory diseases, the concentration of active proteases overwhelms the number of inhibitors, which causes excessive proteolytic tissue degradation, damaging the airway walls and contributing to a destructive inflammatory pathway.
Why chose ProAxsis Respiratory Products?
ProteaseTag® Technology targets only the active form of the protease, thus providing a stronger correlation with pathogenic activity than assays that measure total protease levels (composites of the latent, active and inhibitor-enzyme complexes). Measuring these active proteases in sputum sol shows localized inflammatory activity and response within the respiratory tract.