What is the difference and advantage of the Peviva M30 CytoDeath™ ELISA compared to assays that measure e.g. caspase-3 activity?
The caspase-3 assay measures enzyme activity (with specificity problems associated with “peptide caspase”-substrates), while the M30 CytoDeath™ ELISA measures the accumulation of a caspase cleavage product. Due to the accumulation of the analyte (K18Asp396-NE) over time, M30 CytoDeath™ ELISA is more sensitive and does not require consideration of the optimal analytical (often transient) window. This makes this assay convenient and economical when a large number of samples are analyzed. A limitation of the M30 CytoDeath™ ELISA is that it will only work with epithelial cells.