5450501 Technoclone Technozym ADAMTS13 ELISA measurement assay detection reagent test kit

Technozym® ADAMTS-13 Fluorogenic Activity/Antigen

$0.00

  • Catalog #: 5450501
  • Method: ELISA
  • Packaging: Kit/96 tests
  • Type: Kit
  • Use: Research Use Only (RUO)

Technozym® ADAMTS-13 ELISA is a Fluorogenic ELISA kit for the determination of ADAMTS-13 activity and antigen concentration in human plasma. ADAMTS-13 is the enzyme that cleaves vWF under laminar flow conditions. A functional defect of this enzyme leads to the presence of higher molecular weight forms of vWF and thus to increased platelet aggregation, mainly in the microvasculature.

ELISA test strips (2×6 for 2x48T), with 8 wells each, coated with a monoclonal anti ADAMTS13, directed against the CUB domain; the drying agent is supplied in an aluminium bag.
Washing buffer concentrate (PBS; pH 7.3); containing detergent; 0.01% merthiolate; 1 vial, 100 mL.
Incubation buffer (PBS; pH 7.3); contains stabiliser protein; 0.01% merthiolate; and dye, 1 vial, 100 mL, ready for use.
Calibrators (standards) numbered from 1 to 5; lyophilised; 1 vial each; 0.5 mL. Concentrations are lot-specific; consult the label on the vial or the included batch table.
High and low control plasma lyophilised; 1 vial each; 0.5mL. Concentrations are lot-specific; consult the label on the vial.
Activity substrate 2 vials for 2x48T.; 3 mL each, lyophilised.
Conjugate anti-ADAMTS-13 POX; dyed blue; 1 vial, 0.3 mL.
Antigen substrate 1 vial, 6 mL.
Stable peroxide solution 1 vial, 0.7mL.
Stop solution for antigen substrate 1 vial; 6 mL; ready to use.
Adhesive film for ELISA test strips; 2 pieces.

Advantages

  • Allows determination of ADAMTS-13 activity below 5%
  • Ready-to-use coated ELISA plate
  • Easy to use in-house assay can lead to significant cost savings
  • Plate-based assay with commonly used fluorescent ADAMTS-13 activity substrate
  • Less interferences compared to solution-based fluorescent assays
  • Calibrators and controls included
  • Sample used undiluted
  • Both activity and antigen levels in one assay
  • Excel evaluation software available

Assay Principle

See Graphic

Background

ADAMTS-13
ADAMTS-13 (a disintegrin-like and metalloproteinase with thrombospondin type 1 motif 13) is an enzyme (vWF-cleaving protease or vWF-CP) that specifically cleaves von Willebrand Factor (vWF) multimers.