Technoclone Anti-PAI-1 Monoclonal Antibody 5PAI
IgG1; reaction with active and latent PAI-1 and t-PA-PAI-1 complexes; no cross-reaction with PAI-2 or with PAI-3; interferes with the functional activity of PAI-1.Applications include immunodepletion of PAI-1 from human plasma and immunopurification in ELISA tests and immunohistochemistry.
Binds to active PAI-1, to latent PAI-1, to inactive PAI-1 and to t-PA-PAI-1complexes. No binding to PAI-2 or PAI-3.
Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified PAI-1 from the human melanoma cell line MJZJ according to the method of Wagner et al. (1).
The antibody is purified from murine ascites by ammonium sulphate precipitation followed by affinity chromatography on protein G sepharose. The purity of the product is tested by Laemmli SDS-PAGE.
The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium acide. It is supplied in vials of 500 µg and should be reconstituted with 0.5 ml distilled water. For extensive dilutions a protein containing solution should be used (e.g. 1%bovine serum albumin in PBS). Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.
1) O.F.Wagner, M.Vetterlein, and B.R.Binder: Purification of an Active Plasminogen Activator Inhibitor Immunologically Related to the Endothelial Type Plasminogen Activator Inhibitor from the Conditioned Media of a Human Melanoma Cell Line. J.Biol.Chem. 261: 14474, 1986.