Measure Only ACTIVE Neutrophil Elastase With ProAxsis ELISA
Posted: March 11, 2021
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For ProAxsis ProteaseTag™ NE Assay
Why measure only active NE?
- Neutrophil Serine Proteases (including neutrophil elastase) are important for immune responses to bacterial infections.
- Bacterical infections are a major hurdle for exacerbations in lung diseases like COPD, CF and Bronchiectasis.
- NE is an enzyme that exists in latent, active and inhibitor bound states.
- Higher levels of active NE enzyme vs. total NE in a sample has been shown to correlate with severity of chronic lung diseases and occurrence of bacterial exacerbations.
- Development of NE inhibitors is an attractive therapeutic target for pharmaceutical companies, but requires excess active NE enzyme to be effective.
- This makes measurement of active NE critical during drug development stages.
(Respiratory Research Thulborn, 2019)
ProAxsis tackles the need to measure only active NE
ELISA with ProteaseTag™ technology
ProAxsis (Belfast, Ireland) has tackled this need to specifically measure only active NE by developing an ELISA formatted assay with their ProteaseTag™ technology. In this kit, a NE-specific protease inhibitor acts as a “capture Ab” to immobilize only NE in the active form and free of native biological inhibitors.
The technology is suitable for complex biological samples (ie sputum or BAL) and is not dependent on kinetic readouts for precise, sensitive and reproducible data. This assay is able to distinguish between active and latent NE enzyme and quantitative between 7.2 -1000 ng/mL.
Learn more details about ProAxsis ProteaseTag® Active NE Immunoassay here.
Visit diapharma.com for more.
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