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Technochrom® FVIII:CH is a reagent kit for the photometric determination of coagulation Factor VIII activity in plasma and Factor VIII concentrates by measuring Factor Xa generation with a chromogenic substrate. This kit shows an excellent correlation with one and two stage Factor VIII assays and requires only a single linear calibration curve between 0 and 144%. It contains all components, is insensitive to heparin up to 10 IU/mL and is suitable for plasma and Factor VIII concentrates. It can be used for assaying Factor VIII deficiencies as well as therapy effects.
| mL | Reagent | Contains |
|---|---|---|
| 2×2 | Substrate | 10 µL FXa-1, 0.012 µmol αNAPAP |
| 2×2 | Reagent A | Phospholipid, albumin |
| 2×2 | Reagent B | FIXαβ, FX, Ca2+, albumin, thrombin |
| 1 | Ref Standard 1 | ~130% (1.3 IU) FVIII/mL |
| 1 | Ref Standard 2 | ~70% (0.7 IU) FVIII/mL |
| 1 | Ref Standard 3 | ~10% (0.1 IU) FVIII/mL |
| 1 | Ref Standard 4 | <0.5% (0.005 IU) FVIII/mL |
| 2×20 | FVIII Dilution Buffer | 3.4 g/L Imidazole, 5.85 g/L NaCl, 0.2% albumin, pH 7.4 |
| 2×8 | FVIII Reaction Buffer | 6.06 g/L Tris, 3.03 g/L Na2EDTA, 25 g/L NaCl, pH 8.3 |
| F IIa | ||
| FVIIIsample + (FIXaß-PL- Ca++) | → | FVIIIa-FIXaß-PL-Ca++-complex |
| FVIIIa-FIXaß-PL-Ca++-complex | ||
| FX | → | FXa |
| Substrate-FXa-1 | → | Substrate-FXa-1 + pNA |