Technoclone Purified Glu-Plasminogen, 1mg
$0.00
Glu-Plasminogen purified protein, lyophilized. For use in research tests. Not for drug use. Prepared from pooled human plasma. Each donor is individually tested and found free of hepatitis and AIDS antibodies.
The product is lyophilized from 1% Hepes, 1% Glycin, 1% Saccharose, 2,5% Mannit buffer, pH=6,6 at a protein content of 1 mg/ml.
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Reconstitution
The lyophilized product is reconstituted by addition of 1 ml distilled water and gentle agitation 10 minutes at room temperature followed by centrifugation of 3 minutes at 1000g.
Analysis after reconstitution
| 1 | Appearance | clear, colorless solution |
| 2 | Protein content (1) | 1,2 mg |
| 3 | Glu-Plasminogen (2) | > 90 % |
| 4 | Lys-Plasminogen (2) | < 10 % |
| 5 | Plasmin content (3) | < 0,01 % |
| 6 | activatable by t-PA (3) | 16 IU /ml |
| 7 | activatable by SK (3) | 30 IU/ml |
| 8 | Caseinolytic Units / vial | 20 CU/ml |
| 9 | SDS PAGE (2) | See Image lane A) Marker (Page Ruler) lane B) Glu-Plasminogen RCD03B0 lane C) Lys-Plasminogen, Lot RCG91A0 |
- Spectrophotometrically at 280nm, Factor 1,7
- SDS-PAGE using Phast Gel Gradient 8-25% (Amersham Pharmacia Biotech), silver stain
- Plasmin activity analyzed amidolytically (Kabi S-2251 H-D- Val-Leu-Lys-pNA). Colr development at 405nm was monitored at 37°C.