Technoclot® PT Owren (manual) is a thromboplastin reagent modified according to Owren for the quantitative determination of Prothrombin Time (PT) in human citrated plasma and capillary blood. The reagent is enriched with bovine plasma from which the prothrombin complex factors II, VII and X have been removed. The bovine plasma remains a source of fibrinogen and factor V, which therefore cannot be detected by the test.

Technoclot® PT Owren replaces Thrombotest® and Normotest®.

The reagent is lyophilized and contains rabbit brain thromboplastin and adsorbed bovine plasma. The adsorbed plasma is added as a source of factor V and fibrinogen. Technoclot® PT Owren manual is a combined thromboplastin which already contains CaCl2.

Materials required (not supplied with the kit)

  • Pipettes
    Capillaries 25µl, 2 x 100 pcs, Catalog# 9801025
  • Distilled water
  • Control Plasma*
    Coagulation Control N, 5 x 1 ml, Catalog# 5020040
    Coagulation Control AK, 5 x 1 ml, Catalog# 5011050
    Coagulation Control A, 5 x 1 ml, Catalog# 5021055
    Technoclot® PT Owren Capillary Control Set, Catalog# 5005102
  • Calibration Plasma
    AK-Calibrant, 4 x 1 ml, Catalog# 5010004
    Coagulation Reference, 5 x 1 ml, Catalog# 5220110
    Technoclot® PT Owren Capillary Calibration Set, Catalog# 5005100
    * or any other package sizes or Technoclot® reagent

This reagent is sensitive to abnormal levels of the coagulation factors II, VII and X. It is used for the control of blood coagulation disorders of the extrinsic system as well as the monitoring of oral anticoagulant therapy (i.e. warfarin). Technoclot® PT Owren manual is especially designed to be used with manual methods (i.e. tilting) and semiautomated coagulometers.