Lys-Plasminogen purified protein, lyophilized. For use in research tests. Not for drug use. Prepared from pooled human plasma from blood donors in Vienna. Each donor is individually tested and found free of hepatitis and AIDS antibodies.
The product is lyophilized from 0.1M NaCl, 0.02M phosphate buffer, pH=7.3.
The lyophilized product is reconstituted by addition of 1ml distilled water and gentle agitation 10 minutes at room temperature followed by centrifugation 3 minutes at 1000g.
Analysis of reconstitution
|Appearance||clear, colorless solution|
|Protein content (1)||1mg|
|Glu-Plasminogen (2)||< 10%|
|Plasmin content (3)||0.01%|
|activatable by tPA (3)||0,01 delta OD/min|
|activatable by uPA (3)||0,027 delta OD/min|
|SDS PAGE (2)||
Lane A) Reference mix of Glu- and LysPlasminogen
(1) Spectrophotometrically at 280nm (ratio 280nm/260nm)
(2) SDS-PAGE using Phast Gel Gradient 8-25 (Amersham Pharmacia Biotech), silver stain
(3) Plasmin activity analyzed amidolytically (Kabi S-2251 H-DVal-Leu-Lys-pNA). Color development at 405nm and 37°C
|What is the difference between Glu-plasminogen and Lys-plasminogen? How can the two forms be measured?|
|The native form of plasminogen in plasma has glutamic acid (Glu) at the N-terminal and is termed Glu-plasminogen. Other plasminogen forms generated by the catalytic cleavage of plasmin and containing mostly lysine (Lys) at the N-terminal position are termed Lys-Plasminogen. They can be measured using a chromogenic method based on the different rate of activation of the two forms by urokinase. See Scully MF, Kakkar VV. Measurement of Degraded Forms of Plasminogen in Human Plasma. Thromb Res (1980); 17: 347-352. Note that the Chromogenix plasminogen is at least 95% Glu-plasminogen.|
|Is the plasminogen reagent you sell Glu- or Lys- plasminogen?|
|We sell both forms, though most researchers want Glu-Plasminogen, which is a preparation of at least 95% Glu-plasminogen. See the product pages for detailed information about our plasminogen preparations.|
|The activity of plasmin and plasminogen is expressed in CU. What is this, and what is the equivalent in nkat?|
|CU stands for Casein Units, and is a measure of the proteolytic activity on the substrate casein. For example, a plasminogen reagent when activated to plasmin with streptokinase can show an activity of 7.3 nkat (S-2251™) per CU. 1 mg of plasmin corresponds to 0.20 nkat (S-2251™), or to 0.024 CU.|