Ceveron® TGA RC Low Kit (thrombin generation assay reagent C Low) is a reagent kit for use on the Ceveron® Alpha, Ceveron® t100, or  Ceveron® s100 instruments.  The TGA RC Low trigger reagent is most useful for measurement of

  • Hypercoaguable plasma
  • Changes in the positive feedback loop of the intrinsic pathway activation
  • Researching thrombogeneity of microparticles

Ceveron® TGA RC Low is based on monitoring the fluorescence generated by the cleavage of a fluorogenic substrate by thrombin over time, upon activation of the coagulation cascade in plasma by Ceveron® TGA RC Low trigger.

The trigger is composed of tissue factor and negatively charged phospholipids specially adapted to detect very sensitive changes in the positive feedback loop of intrinsic pathway activation following its activation by the extrinsic pathway. This gives the assay high sensitivity to thrombophilic tendencies.

From the changes in fluorescence over time, the concentration of thrombin (nM) in the sample can be calculated using the respective thrombin calibration curve.  The increase in thrombin concentration over time allows the calculation of the thrombin generation curve and to calculate various thrombin generation parameters.

 

Ceveron TGA Reagent C Low Kit Assay ELISA measurement

  Reagent Description
2x 1mL Ceveron® TGA RC Low Trigger reagent with low concentration of phospholipid micelles containing rhTF in Tris-HEPES-NaCl buffer.  Lyophilized
2x 1.5mL Ceveron® TGA BUF Tris-HEPES-NaCl buffer.  Lyophilized
2x 3mL Ceveron® TGA Sub 1 mM fluorogenic substrate Z-G-G-R-AMC.  Lyophilized
2x 1mL Ceveron® CONT H Human plasma with increased thrombin generation.  Lyophilized
2x 1mL Ceveron® CONT L Human plasma with decreased thrombin generation.  Lyophilized
1x 25mL Calcium Chloride solution, 25 mM CaCl2, 25 mM. Ready-to-Use

Stability and Storage

 

The expiration date printed on the labels is applicable so long as the vials remain unopened.  Stability following reconstitution

Reagent Ceveron® Cooling Block 2o-8oC
Ceveron® TGA RC Low 8 Hours 5 Days
Ceveron® TGA BUF 8 Hours 5 Days
Ceveron® TGA Sub 8 Hours 5 Days
Ceveron® CONT H 4 Hours –
Ceveron® CONT L 4 Hours –
Calcium Chloride solution, 25 mM 7 Days 4 Weeks

 

Normal Ranges:

This example normal range was determined using 100 heathy, normal donor PPP sample.  Each laboratory should establish and control its normal range.

Reagent nM Peak Thrombin nM AUC
Ceveron® TGA RC Low Kit 43-368 1236-2945

Abdominal and thoraco-abdominal aortic aneurysm (AAA/TAA)

thrombin generation assay abdominal thoraco-abdominal aortic aneurysm AAA/TAA

Subjects with abdominal and thoraco-abdominal aortic aneurysm (AAA/TAA) show abnormal high peak thrombin, which correlates with aneurysm size.

 

Preeclampsia and activation of thrombin generation in the third trimester

thrombin generation assay preeclampsia
During the manifestation of the disease, subjects with preeclampsia have significantly higher thrombin generation compared to patients with normal pregnancies.