Ceveron® TGA RC High Kit
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Ceveron® TGA RC High Kit (thrombin generation assay reagent C high) is a reagent kit for use on the Ceveron® Alpha, Ceveron® t100, or Ceveron® s100 instruments. The TGA RC High trigger reagent is most useful for measurement of
- DOACs levels in plasma
- Direct thrombin inhibitor levels in plasma
- Vitamin K antagonists
Ceveron® TGA RC High is based on monitoring the fluorescence generated by the cleavage of a fluorogenic substrate by thrombin over time, upon activation of the coagulation cascade in plasma by Ceveron® TGA RC High trigger.
The trigger is composed of tissue factor and negatively charged phospholipids specially adapted to induce a thrombin burst upon activation of the extrinsic pathway. This allows for high sensitivity of thrombin generation to anticoagulated plasma.
From the changes in fluorescence over time, the concentration of thrombin (nM) in the sample can be calculated using the respective thrombin calibration curve. The increase in thrombin concentration over time allows the calculation of the thrombin generation curve and to calculate various thrombin generation parameters.
| Ceveron® TGA RC High | 2x 1mL | Trigger reagent with high concentration of phospholipid micelles containing rhTF in Tris-HEPES-NaCl buffer. Lyophilized |
|---|---|---|
| Ceveron® TGA BUF | 2x 1.5mL | Tris-HEPES-NaCl buffer. Lyophilized |
| Ceveron® TGA Sub | 2x 3mL | 1 mM fluorogenic substrate Z-G-G-R-AMC. Lyophilized |
| Ceveron® CONT H | 2x 1mL | Human plasma with decreased thrombin generation. Lyophilized |
| Ceveron® CONT L | 2x 1mL | Human plasma with decreased thrombin generation. Lyophilized |
| Calcium Chloride solution, 25 mM | 1x 25mL | CaCl2, 25 mM. Ready-to-Use |
Stability and Storage
The expiration date printed on the labels is applicable so long as the vials remain unopened. Stability following reconstitution:
| Reagent | Ceveron® Cooling Block | 2o-8oC |
|---|---|---|
| Ceveron® TGA RC High | 8 Hours | 5 Days |
| Ceveron® TGA BUF | 8 Hours | 5 Days |
| Ceveron® TGA Sub | 8 Hours | 5 Days |
| Ceveron® CONT H | 4 Hours | – |
| Ceveron® CONT L | 4 Hours | – |
| Calcium Chloride solution, 25 mM | 7 Days | 4 Weeks |
Normal Ranges
This example normal range was determined using 100 heathy, normal donor PPP sample. Each laboratory should establish and control its normal range.
| Reagent | nM Peak Thrombin | nM AUC |
|---|---|---|
| Ceveron® TGA RC High Kit | 84-626 | 1299-3145 |
Thrombin generation detects differences in the coagulation capacity in anticoagulated plasma samples.
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