Technoclone Anti-Plasminogen Monoclonal Antibody 7PG
IgG1; positive reaction with free plasminogen or plasmin in complex with Alpha-2-Antiplasmin. Directed against an epitope on the kringle 4 elastase fragment of plasminogen. Applications include biochemichal and pharmalogical studies.
Positive reaction with free Plasminogen or Plasmin in complex with α-2-antiplasmin complexes. Directed against an epitope on the kringle 4 elastase fragment of plasminogen (1).
Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified human plasminogen.
The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.
The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/ml mannitol. It is supplied in vials of 500µg and should be reconstituted with 0.5 mL distilled water.
For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS).
Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.
1) E. Hattey, J.Wojta, and B.R.Binder: Monoclonal antibodies against plasminogen and α-2-antiplasmin: Binding to native and modified antigens. Thrombosis Research 45: 485,1987