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Recognizes only scu-PA/pro-urkinase, no reaction with two chain urokinase.
Binds specific to single chain urokinase.
Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with recombinant human single chain urokinase.
The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.
The antibody is lyophilized from a 0,5 mg/ml solution in 10 mM bicarbonat buffer, pH 9.6 It is supplied in vials of 500µg and should be reconstituted with 1 ml distilled water.
For extensive dilutions a protein containing solution should be used (e.g. 1% bovineserum albumin in PBS).
Lyophilized antibody should be stored at 4oC. Reconstituted antibody should be aliquoted and stored at -20oC or lower. Avoid repeated freeze-thaw cycles.