Technoclone Anti-scu-PA Monoclonal Antibody 14scu-PA

IgG₁; binds to single chain urokinase, two-chain urokinase, and low molecular weight urokinase. Partially inhibits the functional activity of two-chain urokinase at appropriate concentrations. Applications include immunoblotting, ELISA tests and enzyme inhibition.

Specificity

Binds to single chain urokinase, two-chain urokinase and low molecular weight urokinase. Inhibits the functional activity of two-chain urokinase at appropriate concentrations.

Derivation

Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with recombinant human single chain urokinase.

Antibody class

IgG₁, Kappa light chain

Purification

The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.

Inhibition of urokinase

• 14 scu-PA at concentrations ranging from 0-10 µg/ml was preincubated with 20IU/ml urokinase for 15 minutes at 37°C
• Urokinase activity was detected with a chromogenic substrate (S-2444)
• Optical density was read after 15 minutes incubation with the chromogenic substrate at 37°C

Handling and storage

The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/mL mannitol. It is supplied in vials of 500 µg and should be reconstituted with 0.5ml distilled water.

For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS).

Lyophilized antibody should be stored at 4°C. Reconstituted antibody shouldbe aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.