Technoclone Anti-Plasminogen Monoclonal Antibody 4PG

IgG₁; positive reaction with mini-plasminogen, no reaction with kringles 1-3 or kringle 4 as demonstrated by immunoblotting; reaction with free, but not complexed, plasminogen-plasmin. Applications include biochemical and pharmacological studies and antibody for an ELISA specific for plasminogen.

Specificity

Positive reaction with miniplasminogen, no reaction with kringles 1-3 or kringle 4 as demonstrated by immunoblotting; reaction with plasminogen and free plasmin, but not with plasmin/antiplasmin complexes (1).

Derivation

Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified human plasminogen.

Antibody class

IgG₁

Purification

The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.

Handling and storage

The antibody is lyophilized from a 1mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20mg/ml mannitol. It is supplied in vials of 500µg and should be reconstituted with 0.5ml distilled water.

For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS).

Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.

Literature

1. E. Hattey, J.Wojta, and B.R.Binder: Monoclonal antibodies against four different epitopes of plasminogen exhibit different effects on plasminogen activation kinetics. Thrombosis and Haemostasis 58, No.1: 440, Abstr. 1622, 1987