Technoclone Anti-Alpha2-Antiplasmin 14AP

Technoclone Anti-alpha2-Antiplasmin 14AP

Lyophilized. Monoclonal. IgG_2a; inhibits alpha-2-antiplasmin activity in a functional assay system.

Specificity

Recognizes functional α-2-antiplasmin.

Derivation

Hybridiation of NSO cells with spleen cells from a BALB/c mouse immunized with purified α-2-antiplasmin.

Antibody class

IgG_2a

Purification

The antibody is purified from hybridoma supernatants by affinity chromatography on immobilized recombinant Protein G. The purity of the product is tested by Laemmli SDS-PAGE.

α-2-antiplasmin Inhibition

• 14 AP at 100 µg/ml preincubated for 15 minutes with α-2-antiplasmin at 37°C
• Plasmin addition. Incubated for 15 minutes at 37°C
• Measured uncomplexed plasmin with a plasmin substrate (S-2251) for 15 min at 37°C
• Recorded optical density at 405 nm (OD 405)

Handling and storage

The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/ml mannitol. It is supplied in vials of 500 µg and should be reconstituted with 0.5 mL distilled water.

For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS).

Lyophilized antibody should be stored at 4°C. Recon stituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.