Technoclone Anti-alpha2-Antiplasmin 2AP

Lyophilized. Monoclonal. IgG₁; positive reaction with native alpha-2-antiplasmin and degraded alpha-2-antiplasmin as well as with plasmin-alpha-2-antiplasmin complexes as demonstrated by immunoblotting. Half maximal binding to coated alpha-2-antiplasmin at 9ng/ml. It can be used in an ELISA-system as second antibody.

Specificity

Binds native α-2-antiplasmin and degraded α-2-antiplasmin as well as plasmin-α-2-antiplasmin complexes (1). Half maximal binding to coated α-2-antiplasmin at 9 ng/ml.

Derivation

Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified α-2-antiplasmin (1).

Antibody class

IgG₁

Purification

The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.

Handling and storage

The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/ml mannitol. It is supplied in vials of 500 µg and should be reconstituted with 0.5 mL distilled water. For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS). Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.

Literature

1. E. Hattey, J.Wojta, and B.R.Binder: Monoclonal antibodies against plasminogen and α-2-antiplasmin: Binding to native and m