Collagen is tough (pun intended), make your life easier with these QuickZyme kits

Posted on: May 4, 2023

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It’s Not Just Support. It’s Solutions.

DiaPharma and QuickZyme Know Matrix Matters!

For tissues with lower collagen levels and high matrix effect, analyzing collagen is difficult. QuickZyme therefore developed an assay in which the matrix effect was neutralized to make total collagen analysis suited for more organs or imbedded tissue. The references below are based on the sensitive tissue collagen assay and is focused on use in the liver, but their methods are applicable to a variety of organs, including heart, lung, kidneys and embedded tissue samples.

QuickZymeTotal Collagen Kit – hydroxyproline detection method details:

Compatible with many tissue digestion protocols
Wide detection range for accurate quantitation
Easy 2 hour protocol with no drying step
Application available for low collagen organs and paraffin imbedded tissue
Applicable to all species types

References:

van den Hoek AM, Verschuren L, Worms N, et al. A Translational Mouse Model for NASH with Advanced Fibrosis and Atherosclerosis Expressing Key Pathways of Human Pathology. Cells. 2020;9(9):2014. Published 2020 Sep 1. doi:10.3390/cells9092014

van den Hoek AM, de Jong JCBC, Worms N, et al. Diet and exercise reduce pre-existing NASH and fibrosis and have additional beneficial effects on the vasculature, adipose tissue and skeletal muscle via organ-crosstalk. Metabolism. 2021;124:154873. doi:10.1016/j.metabol.2021.154873

Collagen assays available from QuickZyme Biosciences: