Chromogenic Substrates – It’s in our Blood

Posted: October 21, 2021

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Chromogenic Substrates for your Coagulation, Anticoagulation, and Fibrinolysis Needs

Chromogenic substrate technology is in our blood.

Chromogenic Substrates for QC, Drug Development, R&D, for Anti-Xa Testing (S-2765), Contact Pathway Studies (S-2302), Thrombin Generation (S-2238), and more.

chromogenic substrates Contact Pathway Studies Thrombin Generation

Chromogenic substrates are peptides that react with proteolytic enzymes with the formation of color. Most blood coagulation and fibrinolytic factors belong to the group of serine proteases (enzymes) and their zymogens (proenzymes), making chromogenic substrate technology a useful and routine tool in the hemostasis field.

Optimizing Your Assay: What are Enzyme Specificity and Substrate Selectivity?

SPECIFICITY is a property of the enzyme and describes how restrictive the enzyme is in its choice of substrate. A single serine protease may act on various substrates although at different rates. The most important factor governing the fit of a substrate for an enzyme is the amino acid sequence around the bond to be cleaved. SELECTIVITY indicates the degree to which the substrate is bound to and cleaved by different enzymes, given by the ratio kcat/Km.

Measurements made using chromogenic substrates reflect enzyme activity – one gains knowledge about the activity of an enzyme rather than the amount of protein present as found in an immunological assay.

Many factors go into optimizing a chromogenic substrate assay, such as proper selection of type of buffer, pH, relative concentrations of sample and reagents, addition of inhibitors, and/or choice of activator or incubation times. DiaPharma offers comprehensive overviews of our substrates to serve as a guide in selecting a proper substrate. The scientific team at DiaPharma is also here to assist.

For research use only. Not for use in diagnostic procedures.

prothrombin cleaved by factor xa
Prothrombin, the natural substrate of Factor Xa, is cleaved by Factor Xa at two positions, each proceed by the same four amino acid sequence. Factor Xa activity can be determined by the chromogenic substrate S-2222*, which is composed of the same amino acids coupled to a chromophore.


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