If using a BioTek® fluorescent reader, can I keep the machine on all day?

Unfortunately, no, Technoclone and BioTek® recommend running only 2 consecutive runs and to switch the reader off for ~2-3 hours before starting the next run. The intensity of the lamp begins to diminish after too long. In addition, the bulb should be changed once a year (lifespan is ~500 hours anytime the instrument is on).

I received a BioTek® “Run-time error ‘1004’ Cannot insert object.” Now what?

This can occur with the Gen5 software associated with the BioTek® reader. Some newer versions of Microsoft Excel may be incompatible with older versions of Gen5 Software. Gen5 v1.11 or higher is needed. There is a cost associated with an upgrade to version 2.0, and BioTek® Customer Service must be contacted for this software upgrade. However, Gen5 v1.11 will work and can be downloaded here: https://customer.biotek.com/login/
(Your Gen5 Software serial number will be needed).

I received another BioTek® error when trying to power export. What should I do?

While some issues may require your IT support, in some situations simply saving the data, and then restarting the computer, opening the data, and selecting power export again will fix the error (eg, this may resolve errors related to ‘cannot find path’). In addition, be sure that screen savers are turned off as this can interrupt the experiment.

I receive an error after BioTek® power export, what happened?

It’s likely that the data was not correctly copied into the excel evaluation template (Note: Technoclone.com even provides document instructions on this). Cells must be copied to corresponding cell for data analysis of the calibration curve or samples/controls within the ‘Raw Data’ excel sheet tab. Raw data can then be analyzed with Excel protected formulas.

The BioTek® instrument reading is completed, how do I analyze my data?

You need to select ‘Power Export’. This will automatically open the data in an Excel file. However, as noted above, do not analyze data using standard Microsoft Excel. Your data must be copied and pasted into the Excel Evaluation Template/Software that corresponds to the instrument used.

I have created the protocols for the calibration curve and samples/controls, now what?

Prepare for running the experiment by opening the fluorescent reader software, creating a new experiment, and selecting the appropriate protocol. For some instruments, pre-heating should begin right away. To run the actual experiment, follow the short instructions provided with the Technothrombin® TGA for the calibration curve and samples/protocol. At some point, the plate layout within the Excel Evaluation Software/Template should be completed in order to help with subsequent data analysis.
Note: Only one calibration curve has to be done for each lot of substrate.

How do I set-up the protocol on the BioTek® instrument?

Protocol set-up on the instrument software only needs to be completed once. Two protocols are required: one for the calibration curve and one for running the samples and controls. Instrument read settings are critical! Technoclone maintains instrument application documents for the available instruments. The instrument application documents contain screenshots to help with set-up but the overall basic parameters settings will remain similar if the instrument software is ever updated.

Where is the TGA evaluation software?

The manufacturer refers to the Microsoft Excel data analysis templates as evaluation software. These are templates created by Technoclone that can be used for data analysis with a number of fluoresecent readers. The excel templates are available at www.technoclone.com or you can request one at info@diapharma.com.

Do not analyze your data with standard Microsoft Excel.

What type of Fluorescent Reader do I need?

While the manufacturer currently recommends using the Ceveron® alpha TGA analyzer, alternative fluorescent readers can be used. Excel data evaluation templates have been created for the BioTek® FLx800™, Spectramax M5, BMG FLUOstar, Perkin Elmer Wallac Viktor, TECAN, and Thermo Fluoroskan.