TGA Testing Products
Thrombin Generation Assay (TGA) is a more global downstream read out for the kinetics of thrombin generation during initiation, amplification and down regulation of thrombin formation from activation of proteins in the coagulation cascade, like PKA and FXIa. Technothrombin® TGA is a complete fluorogenic tool for a broad range of applications and is therefore a universal assay kit for analyzing and researching the function of the hemostatic system.
Thrombin Generation Assays
| Technothrombin TGA | Ceveron TGA | Calibrated Automated Thrombogram | Thrombodynamics 4D | |
|---|---|---|---|---|
| Hands-On Time | 30 minutes | <5 minutes | 30 minutes | <5 minutes |
| Assay Time | 60 minutes | 40 minutes | 60 minutes | 60 minutes |
| Complexity to Operate | Standard Laboratory Skills and Equipment | Basic Laboratory Skills and Equipment | Standard Laboratory Skills and Equipment | Single-Button Operation |
| Barcode | No | Yes | No | Yes |
| Plasma Volume | 40 µL/Reaction | 40 µL/Reaction | 80 µL/Reaction | 120 µL/Reaction |
| Calibration Required | Yes, curve | Yes, curve | Yes, curve | No |
| Individual α2M Calibrator | Not Needed | Not Needed | Necessary for Every Sample | Not Needed |
| Total Volume | 100 µL | 150 µL | 120 µL | 125 µL |
| Assay Format | 96 Well Plate | Cuvette | 96 Well Plate | 2 Channel Cuvette |
| Throughput | ~48 Samples/Hour | ~48 Samples/Hour | ~48 Samples/Hour | 2 Samples/Hour |
| Analysis | Excel Application | Ceveron Alpha | Thrombinoscope | Thrombodynamics |
| Reaction | Homogenous | Homogenous | Homogenous | Propagation from surface |
| Trigger | Variable | Variable | Variable | Variable |
| Thrombin Generation Parameters | Lag Time (min) | Lag Time (min) | Lag Time (min) | Lag Time (min) |
| Peak Thrombin (nM) | Peak Thrombin (nM) | Peak Thrombin (nM) | Peak Thrombin (AU/L) | |
| Velocity Index | Velocity Index | Time to Peak (min) | Time to Peak (min) | |
| Total Thrombin Potential | Total Thrombin Potential | Endogenous Thrombin Potential | Total Thrombin Potential (AU*min/L) | |
| Rate of Thrombin Propagation (µm/min) | ||||
| Amplitude of Stationary Thrombin Peak (AU/L) | ||||
| Additional Parameters | Clotting Assays | SpatioTemporal Dyanmics of Fibrin Propagation | ||
| Chromogenic Assays | Clot Size (µm) | |||
| Turbidimetric Assays | Clot Density (a.u.) |
TGA COMPARISON
Research use only in the US and Canada
ML-00-00424REV01