IQ Products offers antibodies to detect various types of hemoglobin. Each antibody can be used individually or combined in a panel.
Anti-hemoglobin S FITC
This is the first anti-hemoglobin S (HbS) antibody conjugate available for flow cytometry. For your convenience, it’s conjugated with FITC, so it can be measured on all available flow cytometers.
The anti-HbS antibody recognizes the abnormal β-chain of HbS. Substitution of the amino acid valine for glutamic acid in the sixth position of the β-chain results in the formation of a βs subunit. Due to the mutated subunit βs, hemoglobin becomes unstable when no oxygen is bound, resulting in sickle-shaped erythrocytes.
Figure 1. Flow cytometry analysis of whole blood stained with anti-hemoglobin S FITC. Histogram: uninterrupted line: stained HbShigh whole blood sample. Interrupted line: stained normal whole blood sample. Dotplot: stained HbShigh whole blood sample.
Anti-pan hemoglobin APC
The murine antibody against human pan hemoglobin (panHb) recognizes the α-chain of human hemoglobin. This antibody can be combined with other antibodies to determine the percentage of erythrocytes containing pathological forms of hemoglobin compared to the total erythrocyte population.
Figure 2. Flow cytometry analysis of normal whole blood stained with anti-pan hemoglobin APC. Histogram: uninterrupted line: stained normal whole blood sample. Interrupted line: blanc sample. Dotplot: stained normal whole blood sample.
Anti-hemoglobin F Dy-410
Anti-hemoglobin F antibody recognizes the γ-chain of hemoglobin F (HbF). HbF is predominantly expressed by fetal erythrocytes. In some cases, HbF is also expressed by adult erythrocytes. F-cells can represent up to 25% of the total erythrocyte population.
Figure 3. Flow cytometry analysis of normal whole blood with a 3% spike of commercial cord blood stained with anti-hemoglobin F Dy-410. Histograms: uninterrupted line: stained whole blood sample with a 3% spike of HbFhigh whole blood, interrupted line: unstained whole blood sample with a 3% spike of HbFhigh whole blood.
Co-staining samples with anti-HbS and anti-HbF antibodies
In many cases, the anti-HbS and anti-HbF antibodies will be used in combined staining to identify double-positive cells. Results from such a double staining are displayed in figure 4. The double staining was performed on normal blood (AA), cord blood (CB), and HbShigh and HbFhigh blood (SF). The AA sample shows no staining with the anti-HbS antibody and a small population of HbF+ cells, the F cells. Staining of the CB sample also resulted in no signal with the anti-HbS antibody and a 96% HbFHigh population. The small population in the lower left quadrant reflects the initiated transition towards HbA in the sample. The anti-HbS antibody stained 98.4% of all red blood cells in the SF sample, with 67.8% being double positive for HbF.
Figure 4. Flow cytometry analysis of whole blood samples stained with anti-hemoglobin S and anti-hemoglobin F. AA: normal adult blood, CB: cord blood, and SF: HbShigh and HbFhigh blood. Data kindly provided by TransLab, Boston Children’s Hospital, USA.
Product information
| Item | Clone | Description | Regulatory status | Package size | Product code |
| Anti-HbS FITC | 57-8 | Anti-hemoglobin S (HbS) antibody conjugated with fluorochrome FITC | [ASR] |
1.0 mL (50 µg/mL) | IQP-574F |
| Anti-HbF1 Dy-410 | WBAC HbF1 | Anti-hemoglobin F (HbF1) antibody conjugated with fluorochrome Dy-410 | [ASR] |
1.0 mL (50 µg/mL) | IQP-567D |
| Anti-HbF1 FITC | WBAC HbF1 | Anti-hemoglobin F (HbF1) antibody conjugated with fluorochrome FITC | [ASR] |
1.0 mL (50 µg/mL) | IQP-567F |
| Anti-HbF1 R-PE | WBAC HbF1 | Anti-hemoglobin F (HbF1) antibody conjugated with fluorochrome R-PE | [ASR] |
1.0 mL (50 µg/mL) | IQP-567R |
| Anti-panHb APC | PHB1 | Anti-pan hemoglobin (panHb) antibody conjugated with fluorochrome APC | [ASR] |
1.0 mL (50 µg/mL) | IQP-575A |
[ASR] Analyte Specific Reagent, analytical and performance characteristics are not established. The product complies with the ASR definitions of the U.S. Food & Drug Administration (FDA).
[RUO] Research Use Only