Rox Prothrombin
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Rox Prothrombin is a chromogenic assay kit for the quantitative determination of Prothrombin (FII) functional activity in plasma and FII containing concentrates. The method is suitable for plasma collected in citrate or EDTA.
- A chromogenic Prothrombin method based upon the prothrombinase complex.
- non-γ-carboxylated-FIIis not activated in the method in contrast to snake venom based prothrombin methods.
- Detection Limit = about 0.05 U/mL (5%) when using a plasma dilution of 1:200 as prescribed in the package insert. The detection limit for undiluted samples is about 0.25 mU/mL.
| Activator Reagent, 3.0 ml (4 vials) (catalog# 2010) |
The Activator Reagent contains lyophilized human FXa, bovine FVa, CaCl2 and phospholipids. Each vial is sufficient for 25 tests. |
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| FIIa Substrate, 6 ml (1 vial) (catalog# 2080) |
Liquid solution of a chromogenic FIIa substrate (H-D-Phe-Pip-Arg- pNA), 2.0 mmol/L. |
| FII Diluent Buffer, Stock Solution, 20 ml (1 vial) (catalog# 2050) |
Liquid stock solution of diluent buffer. |
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Measurement Principle
FII functional activity is determined in a chromogenic prothrombinase method, in which human FII is activated to thrombin (FIIa) by human FXa in the presence of bovine FV, calcium ions and phospholipid.
The amount of FIIa formed is determined from the hydrolysis of a chromogenic FIIa substrate. The FII activity of the sample is assigned vs. plasma or a FII concentrate standard with FII potency expressed in International Units (IU).
The prothrombinase complex is sensitive to g-carboxylation and therefore non-γ-carboxylated-FII is not activated in this method in contrast to snake venom based prothrombin methods.
Background
Factor II is a single chain vitamin K dependent glycoprotein of 72 kDa, which is activated to thrombin (FIIa) by FXa in the presence of FVa, calcium ions and phospholipids.
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