QuickZyme Total Collagen Assay Kit

Application: The assay is used for the measurement of total collagen. This includes all procollagen, unfolded collagen, mature collagen as well as collagen degradation products of all collagen types present in the sample. Since the first step is complete hydrolysis of the sample, difficulty in extraction of collagen plays no role. The assay is applicable for all types of samples, including tissue.

Also available in 5x 96-assays bulk pack (QZBtotcol5)

If you already have a hydrolysis protocol, the Hydroxyproline Assay Kit (link).

The Total Collagen assay kit is based on the quantitative colorimetric determination of hydroxyproline residues, obtained by acid hydrolysis of collagen. Usually for this analysis upon hydrolysis removal of HCl is needed, which is a time-consuming step and / or needs special equipment. The QuickZyme Total Collagen assay is the only hydroxyproline-based assay which does not need this laborious step, resulting in a fast (<2 hrs) and easy (96-well plate format) assay following the hydrolysis step.

Each 96 test kit includes:

• 100 special screw-capped tubes needed for sample hydrolysis
• 2 Adhesive plate seals
• Assay buffer
• 2x Detection reagent A
• Detection reagent B
• Collagen standard (1200 μg/ml) in 0.02M acetic acid
• 96-well assay plate
• Assay protocol booklet

The following materials and equipment are required but not supplied:

• 12M and 6M HCl for sample hydrolysis
• 4M HCl for sample and standard dilution
• Single and/or multichannel pipettes
• Eppendorf centrifuge
• Incubator (or thermoblock or calibrated oven) for heating at 95oC (not higher!)
• Incubator for heating at 60°C
• Microplate reader capable of measuring at a wavelength between 540 and 580 nm, 570 nm preferred.
• Microplate shaker
• PP-, PE- or glass tubes (no polystyrene)

Quantitative measurement of all types of collagens, species independent.

Samples: Cellular extracts, tissue homogenates, tissues.

Colorimetric measurement of hydroxyproline content

Range: 6 to 300 μg/ml.

Sensitivity: 2.5 µg/ml

Uses rat tail collagen as standard

Ease-of-use: Equivalent to ELISA

Run time: < 2 hours

Store at room temperature