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MMP-14, also known as MT- MMP or MT1-MMP, is a membrane type MMP and contains a transmembrane domain. MMP-14 has a role in the activation of the proforms of MMP-2 and MMP-13. Furthermore, MMP-14 is able to directly degrade certain matrix proteins such as laminin, fibronectin, vitronectin and interstitial collagens. Human MMP-14 consists of 562 amino acid residues including a propeptide of 91 residues. and is most likely intracellularly activated by furin-like enzymes and transported to the membrane as an active enzyme. MMP-14 is thought to be released from the cell membrane by proteolytic cleavage. The QuickZyme human MMP-14 activity assay enables you to specifically measure active human MMP-14. It can be used for the measurement of MMP-14 activity in various biological samples, such as cultured cells and tissue homogenates.
MMP-14 is believed to be involved in bone and tooth development, tumor cell invasion and cell migration and in shedding of a variety of cell surface proteins. Recent work points to an involvement of MMP-14 in plaque vulnerability. A variety of studies show MMP-14 as a biomarker for metastasis and fibrosis in the liver.
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Active MMP-14 Measurement of active human MMP-14 Standards, controls and biological samples are pipetted into the pre-coated plate. Human MMP-14 present in the biological sample is captured by the antibody. After washing, the pro-detection enzyme is added. This is activated by the active MMP-14 into an active detection enzyme. The active detection enzyme is able to cleave the chromogenic substrate, resulting in generation of a yellow color that can be measured at 405 nm using an ELISA plate reader.
96 well microwell plate | 12×8 well non-coated |
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Blocking buffer | 15 ml ready to use |
Antibody stock solution | 20 µl 5.9 mg/ml |
Assay buffer | 30 ml bottle contains 20 ml ready-to-use Tris-HCl buffer |
Extraction buffer | 125 ml bottle contains 100 ml ready-to-use Tris-HCl buffer with Triton-X-100 |
Standard | tube contains 50 µl of 640 ng/ml MMP-14 (human); store at -70°C |
Detection enzyme | tube contains 600 µl detection enzyme in Tris-HCl buffer; store at -20 °C |
Substrate | tube contains 1000 µl peptide substrate in demineralized water |
Wash buffer | 30 ml bottle contains 20 ml 25x concentrated phosphate buffer |