Technochrom® C1-INH
$0.00
The Technochrom® C1-INH kit is a chromogenic C1 Esterase Inhibitor Assay.
For research use in hereditary angioedema (HAE). The C1-esterase inhibitor (C1-INH) is a regulatory protein that functions as an inhibitor of several serine proteases in the complement system, the kallikrein-kinin system, the coagulation cascade and in fibrinolysis.
Assay C1 INH activity in concentrates
- Used for calibration of the International Standard for purified concentrate standard (for batch release testing of therapeutic products)
Assay C1 INH activity in plasma
- Uses coagulation reference plasma calibrated against IS for plasma
For fully automated, bar-coded reagents, please see the Ceveron® C1-INH Assay (5345005) for use on the Ceveron® s100 analyzer.
| Reagent | Size | Description |
| SUB | 1x3mL | Substrate C1-1, 18 µmoL, AcOH. C2H5CO-lys ( ∈ -Cbo)-Gly-Arg-pNA, p.m. = 729.8 |
| C1 | 1x3mL | C1-Esterase, human |
| REF | 1 vial | Coagulation Reference for C1-INH, 125% = 1.25 IU/mL C1-INH (See label for reconstitution volume) |
| CON A | 1x1mL | Coagulation Control A for C1-INH, lyophilized abnormal plasma |
| CON N | 1x1mL | Coagulation Control N for C1-INH, lyophilized normal plasma |
| BUF A | 1x25mL | Sample Buffer A, Tris (6.1 g/L)-NaCI (15 g/l)-Buffer pH 7.4 |
| BUF B | 1x20mL | Reaction Buffer B, Tris (6.1 g/L)-NaCI (15 g/l)-Buffer pH 8.5 |
Advantages
- Assay linearity 20 -120%
- Simple test performance
- Heparin insensitive
- Excellent accuracy and precision
- Adaptation sheets for several analyzers are available
Measurement Principle
C1-inh is titrated against an excess of C1-esterase to form an inhibitory complex, and the residual C1-esterase activity is measured using a chromogenic substrate.
C1-Esterase + C1-INH ⎯⎯→ [C1-Esterase.C1-INH] + C1-Esterase
((rest)
(excess)
C2H5CO-Lys(∈ -Cbo)- (C1-Esterase) C2H5CO-Lys(∈ -Cbo)-
Gly-Arg-pNA + H2O ⎯⎯⎯⎯→ Gly-Arg-OH + pNA”
Background
The C1 esterase inhibitor protein is a normal constituent of serum which functions as a serine proteinase inhibitor of the serpin family.
The C1 esterase inhibitor inhibits the complement proteases C1r and C1s, as well as the proteases Kallikrein, factor XIa, XIIa and plasmin of the blood clotting system.