Ceveron® TGA RC High Kit (thrombin generation assay reagent C high) is a reagent kit for use on the Ceveron® Alpha, Ceveron® t100, or Ceveron® s100 instruments.  The TGA RC High trigger reagent is most useful for measurement of

  • DOACs levels in plasma
  • Direct thrombin inhibitor levels in plasma
  • Vitamin K antagonists

Ceveron® TGA RC High is based on monitoring the fluorescence generated by the cleavage of a fluorogenic substrate by thrombin over time, upon activation of the coagulation cascade in plasma by Ceveron® TGA RC High trigger.

The trigger is composed of tissue factor and negatively charged phospholipids specially adapted to induce a thrombin burst upon activation of the extrinsic pathway.  This allows for high sensitivity of thrombin generation to anticoagulated plasma.

From the changes in fluorescence over time, the concentration of thrombin (nM) in the sample can be calculated using the respective thrombin calibration curve.  The increase in thrombin concentration over time allows the calculation of the thrombin generation curve and to calculate various thrombin generation parameters.

Ceveron TGA Reagent C Low Kit Assay ELISA measurement

  Reagent Description
2x 1mL Ceveron® TGA RC High Trigger reagent with high concentration of phospholipid micelles containing rhTF in Tris-HEPES-NaCl buffer.  Lyophilized
2x 1.5mL Ceveron® TGA BUF Tris-HEPES-NaCl buffer.  Lyophilized
2x 3mL Ceveron® TGA Sub 1 mM fluorogenic substrate Z-G-G-R-AMC.  Lyophilized
2x 1mL Ceveron® CONT H Human plasma with increased thrombin generation.  Lyophilized
2x 1mL Ceveron® CONT L Human plasma with decreased thrombin generation.  Lyophilized
1x 25mL Calcium Chloride solution, 25 mM CaCl2, 25 mM. Ready-to-Use

Stability and Storage

 

The expiration date printed on the labels is applicable so long as the vials remain unopened.  Stability following reconstitution

Reagent Ceveron® Cooling Block 2o-8oC
Ceveron® TGA RC High 8 Hours 5 Days
Ceveron® TGA BUF 8 Hours 5 Days
Ceveron® TGA Sub 8 Hours 5 Days
Ceveron® CONT H 4 Hours –
Ceveron® CONT L 4 Hours –
Calcium Chloride solution, 25 mM 7 Days 4 Weeks

 

Normal Ranges:

This example normal range was determined using 100 heathy, normal donor PPP sample.  Each laboratory should establish and control its normal range.

Reagent nM Peak Thrombin nM AUC
Ceveron® TGA RC High Kit 84-626 1299-3145

Low Molecular Weight Heparins

Ceveron instrument enoxaparin tinzaparin thrombin

Rivaroxaban

thrombin generation assay rivaroxaban

Dabigatran, Rivaroxaban, Apixaban

thrombin generation assay dabigatran rivaroxaban apixaban

Thrombin generation detects differences in the coagulation capacity in anticoagulated plasma samples.