Technoclone Anti-alpha2-Antiplasmin 2AP

Lyophilized. Monoclonal. IgG1; positive reaction with native alpha-2-antiplasmin and degraded alpha-2-antiplasmin as well as with plasmin-alpha-2-antiplasmin complexes as demonstrated by immunoblotting. Half maximal binding to coated alpha-2-antiplasmin at 9ng/ml. It can be used in an ELISA-system as second antibody.

Specificity

Binds native α-2-antiplasmin and degraded α-2-antiplasmin as well as plasmin-α-2-antiplasmin complexes (1). Half maximal binding to coated α-2-antiplasmin at 9 ng/ml.

Derivation

Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified α-2-antiplasmin (1).

Antibody class

IgG1

Purification

The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.

The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/ml mannitol. It is supplied in vials of 500 µg and should be reconstituted with 0.5 mL distilled water. For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS). Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.

1) E. Hattey, J.Wojta, and B.R.Binder: Monoclonal antibodies against plasminogen and α-2-antiplasmin: Binding to native and m