Chromogenix Factor Xa. 71 nkat, is for the determination of Heparin in plasma. Factor X is purified from a barium cytrate elution of bovine plasma and it is activated to FXa by a Russell’s Viper Venom matrix.
For laboratory use only.
Packaging, Storage and Stability
- Factor Xa: 71±10 nkat S-2222™
- Bovine Albumin: 20 mg
- Polyethyleneglycol 8000: 10 mg
- Sodium Chloride: 10 mg
- Tris (hydroxymethyl) aminomethane: 6.4 mg
- Tris (hydroxymethyl) aminomethane HCI: 69 mg
The lyophilized powder is stable at 2-8°C until the expiry date printed on the label. The reconstituted Factor Xa is stable for 1 month at 2-8°C or 6 months at -20°C when used for the determination of Heparin, according to the test conditions of the Chromogenix® Coatest® Heparin kit, catalog # K255539 (25553963). Reconstitute with 10 ml sterile water to obtain an activity of 7 nkat/ml (reaction buffer: 0.05 mol/l Tris buffer pH 8.4).
Chromogenix Factor Xa can be used for anti-FXa chromogenic assays.
The activity of FXa can be measured using the following chromogenic substrates:
|The Chromogenix Factor Xa reagent says it is 71 nkat, but I need to know what that is in g/ml and mol/ml.|
|One katal (kat) is the amount of enzyme that converts one mole of substrate per second. Activated enzymes from Chromogenix such as FXa and thrombin are measured in nkat. 1 nkat = 1 x 10-9 mol of product released per second. The conversion is as follows:
FXa has a MW approximately 44,000.
The specific activity for FXa is 1.9 nkat/mg, as determined with chromogenic substrate S-2222™.
This gives that 71 nkat corresponds to 37.4 mg FXa (=37.4 x10-6 g FXa)
This corresponds to 8.5 x 10-10 mol = 0.85nmol.
The concentration in mol/l and g/l will depend on the dilution volume you choose.
Note: 1 IU corresponds to 20 nkats, which means 71 nkatS-2222 = 3.55 IU.