Chromogenix Coatest® APC™ Resistance – V is an APTT-based assay kit for the screening of factor-V-related APC resistance. The high sensitivity and specificity of the test for the factor V:Q506 mutation is obtained by prediluting the sample plasma with an excess of V-DEF Plasma bioreagent. The test design makes it possible to discriminate between heterozygous and homozygous factor V genotypes. It also allows for analysis of plasma from patients on heparin or oral anticoagulant therapy. High discrimination between genotypes with 100% sensitivity for FV:Q506. Reduces need for PCR determination. Applicable to anticoagulant treated patients.

A time- and money-saving alternative to individual genetic testing, Coatest© APC™ Resistance testing is an ideal solution for physicians and labs. Each kit was designed to be easy to use for in-house screening, offering fast results and low costs for both large- and small-volume labs. The most common FV Leiden screening test performed, it offers unmatched sensitivity for the FV:Q506 mutation – close to 100% specificity – and is applicable to patients on heparin or warfarin.

 

One volume of plasma is prediluted with four volumes of V-DEF Plasma. The dilution is then incubated with the APTT reagent for a standard period of time. Coagulation is triggered by the addition of CaCl2 in the absence and presence of exogenous APC and the time for clot formation is recorded.

Activated Protein C (APC) is a regulator of the coagulation cascade, by specifically inactivating factors Va and VIIIa, in the presence of phospholipids and calcium. In most of the cases (more than 90%), Activated Protein C Resistance (APCR) phenotype is caused by a Factor V gene mutation (Factor V Leiden). The mutation, located on Factor V exon 10 (1691 G –> A), of arginine to glutamine on position 506, renders Factor Va resistant to the cleavage by Activated Protein C. This genetic anomaly can be evidenced with a clotting method performed in the presence or the absence of Activated Protein C.

 

apc resistance v leiden clotting assay test kit

Reagents and their stability when opened

ReagentSizeStabilityTemp
APC/CaCl24×2 ml
5 days
8 hours
2-8°C
15-25°C
APTT reagent1×16 ml1 week
1 month
15-25°C
2-8°C
CaCl21×8 ml1 month
1 week
2-8°C
15-25°C
V-DEF Plasma4×4 ml8 hours
24 hours
3 months
15-25°C
2-8°C
-20°C
APC/CaCl24×2 ml3 months-20°C
Control Plasma Level 11×1 ml6 hours
3 months
2-25°C
-20°C
Control Plasma Level 21×1 ml6 hours
3 months
2-25°C
-20°C

Determinations per kit: 80 – 160

IDEAL FOR:

  • Labs looking to bring testing in-house
  • Labs looking for cost savings
  • Ordering physicians looking for a sensitive assay with rapid turnaround
  • Saves the lab from having to perform genetic test on all patients
  • Economical alternative to genetic testing

FV:Q506 mutation screening apc resistance assay test kit

The Chromogenix Coatest APC Resistance V method was used for analysis of plasma from various sample categories showing discrimination between normal and mutated factor V genotypes.

OAC = Oral Anticoagulant Therapy

Activated Protein C (APC) is a regulator of the coagulation cascade, by specifically inactivating factors Va and VIIIa, in the presence of phospholipids and calcium.

In most of the cases (more than 90%), Activated Protein C Resistance (APCR) phenotype is caused by a Factor V gene mutation (Factor V Leiden). The mutation, located on Factor V exon 10 (1691 G –> A), of arginine to glutamine on position 506, renders Factor Va resistant to the cleavage by Activated Protein C. This genetic anomaly can be evidenced with a clotting method performed in the presence or the absence of Activated Protein C.

Activated Protein C Resistance is tested by using a clotting method performed with or without Activated Protein C.

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