DiaPharma Human Thrombin

DiaPharma Human Thrombin is for use us in all research studies and protocols where a source of highly purified human Thrombin is necessary. DiaPharma Human Thrombin is for in vitro use only. For research use only. Not for use in diagnostic procedures.

When tested in the optimized conditions (0.05M Tris buffer at pH 8.4, containing 0.3M NaCl), the following specific activities are observed:

Chromogenic activity on specific substrate (expressed in nKats/μg):

The exact activity in nKats/μg with S‐2238 is reported on the analysis certificate for each lot.

Origin

Prepared from a prothrombin complex concentrate extracted from human citrated plasma and fully activated in a purified system. Lyophilized from 1 mL of: 10mM MES, 150mM NaCl, pH 6.5 with PEG 6000 @ 20mg/mL and BSA 10mg/mL. Highly purified, mainly in the α form, with a specific activity of more than 2,000 NIH/mg.

Presentation

Thrombin activity is reported in harmonized NIH or IU or USP units by reference to the WHO/NIBSC International Standard for Human Thrombin (01/580) (refer to “A unification of the US (“NIH”) and International Unit into a single standard for Thrombin”, Longstaff et al, Thromb Haemost 2005, 93:261‐6)). NIH is a clotting unit for thrombin activity.

Stabilized and lyophilized human Thrombin.

Vial containing approximately 100 NIH of thrombin.

Reconstitution

Each vial must be restored with 1 ml distilled water, in order to get a concentration of about 100 NIH/ml. Dilute in the adequate buffer, if necessary.

Viral Safety

Any product of biological origin must then be handled with all the required cautions, as being potentially infectious.

Stability

Restored:

• 7 days at room temperature (18‐25°C).
• 21 days at 2‐8°C.
• 6 months at ‐30°C or below.

Lyophilized: Until the expiration date printed on the vial.