DiaPharma’s Human Serum Liver-FABP (FABP1) ELISA Kit is a sandwich-type ELISA kit, provided by DiaPharma Group. The kit is supplied in a standard 96-well format with 12 ready-to-use pre-coated 8-well removable strips. All reagents including buffers and a native 2x Human L-FABP standard are also supplied with the kit.
RESEARCH USE ONLY, NOT FOR DIAGNOSTIC USE.
Product Specifications:
Upper limit of detection: 25000 pg/mL
Lower limit of detection: 100 pg/mL
Sample volume: 100 uL
Antibodies: Capture, Monoclonal Detection, Monoclonal
Standard: Native human protein
Assay time: 3.5 hours
Samples: Serum, plasma, urine, cell culture supernatant
Format: Standard ELISA
Cross Reactivity: Human, Monkey, Swine
RESEARCH USE ONLY, NOT FOR DIAGNOSTIC USE.
The Human Serum L-FABP ELISA Kit is for the quantitative determination of human L-FABP in serum, plasma, urine, and cell culture supernatant. L-FABP is a low molecular soluble protein (14kDa) expressed in the hepatocytes of the liver, the proximal tubule in the kidney, and enterocytes in the intestine. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels.
L-FABP is a sensitive marker for cell damage of liver cells in vitro and in vivo making it a good option for use as a NASH biomarker or DILI biomarker.
NASH: Serum L-FABP levels are elevated in NASH and have been shown to correlate with the degree of fibrosis and inflammation. L-FABP levels may aid as a non-invasive marker in determining the severity of fibrosis and inflammation in research with NASH.
Toxicology/DILI: L-FABP has also been recognized as a biomarker for safety testing in DILI research. L-FABP has superior characteristics regarding tissue distribution and kinetics compared to ALT.
