Technoclone Anti-Vitronectin Monoclonal Antibody 2VN

IgG; binds to human vitronectin. Applications include ELISA tests and immunoblotting.

Specificity

Binds to human Vitronectin.

Derivation

Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified human Vitronectin.

Antibody class

IgG

Purification

The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.

Handling and storage

The antibody is lyophilized from a 1mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20mg/ml mannitol. It is supplied in vials of 500µg and should be reconstituted with 0.5ml distilled water.

For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS).

Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.