Technozym® ADAMTS-13 Activity is a chromogenic ELISA kit for the determination of ADAMTS-13 activity in human plasma. ADAMTS-13 is an enzyme that specifically cleaves unusually large von Willebrand Factor multimers, which induce platelet thrombus formation under high shear stress. If the activity of ADAMTS-13 is lowered for some reason, unusually large vWF multimers may accumulate.

  • Easy to use in-house assay can lead to significant cost savings
  • No need for any special technique or instrument, just standard ELISA equipment
  • ADAMTS-13 activity sensitivity down to 0.5% of normals
  • Short assay time (3-4 h)
  • Calibrators and controls included
  • No interferences from bilirubin, hemoglobin or elastase

Reagents, Composition, Storage and Stability

  • ELISA test strips (12), with 8 wells each, coated with a monoclonal anti GST- Antibody. The drying agent is supplied in an aluminium bag. Stability after reconstitution: expiry date.
  • GST-vWF73 Substrate; 2 vials; lyophilized; 6 mL. Stability after reconstitution: 6 weeks.
  • Calibrators (standards) numbered from 1 to 6; lyophilized; 1 vial each; 0.5mL. Stability after reconstitution: 6 months. Concentrations are lot-specific; consult the label on the vial.
  • High and low control plasma, lyophilized, 1 vial each, 0.5 mL. Stability after reconstitution: 6 months. Concentrations are lot-specific; consult the label on the vial.
  • Reaction buffer; 1 vial; 30 mL; ready to use. Stability after reconstitution: 6 months.
  • Conjugate: HRP conjugated monoclonal anti-N10 Antibody: 1 vial; 12mL; ready to use. Stability after reconstitution: 1 month.
  • Color reagent TMB (Tetramethylbenzidine); 1 vial; 12mL; ready to use. Stability after reconstitution: expiry date.
  • Wash Buffer concentrate, 10-fold concentrated, 1 vial, 32mL. Stability after reconstitution: 6 months.
  • Stop solution; sulphuric acid 0.5mol/L, 1 vial, 12 mL; ready to use. Stability after reconstitution: expiry date.
  • Sample dilution Microplate, 1 plate (ONLY for sample dilution!).

The expiry date printed on the labels applies to storage of the unopened vial at 2-8 °C.

ADAMTS13 Thrombotic Thrombocytopenia

ADAMTS-13

ADAMTS-13 (a disintegrin-like and metalloproteinase with thrombospondin type 1 motif 13) is an enzyme (vWF-cleaving protease or vWF-CP) that specifically cleaves von Willebrand Factor (vWF) multimers.