DISCONTINUED. REPLACEMENT: MP0230
This product has been discontinued in the North American market. If you have interest in purchasing a legacy system or compatible reagents, please contact us.
Multiplate® COLtest is a reagent kit used for collagen induced aggregation in research of platelet aggregation studies for the evaluation of normal platelet function on the Multiplate® Analyzer. Sensitive to a cyclooxygenase inhibition, GpIIb/IIIa antagonists and deficiency of GpIIb/IIIa receptors. For the research of qualitative platelet function inhibition.
Reagents, Composition, Storage and Stability
- MP0193: Multiplate® COLtest reagent, Kit, 1 x 1.0 mL. 50 tests. Reagent is stable until the expiration date printed on the vial label when stored at 2-8°C. Stable 7 days after reconstitution. Do not freeze the reconstituted reagent.
The COLtest leads to platelet activation via the platelet collagen receptor. For suitable platelet activation, the release of endogenous arachidonic acid from the platelet phospholipids is necessary. The arachidonic acid is then transformed to the platelet agonist thromboxane A2 (TXA2) by the enzyme cyclooxygenase. Cyclooxygenase is blocked by Aspirin®. Therefore, the COLtest is sensitive to Aspirin® action. This mechanism also explains why the COLtest is less specific towards the action of Aspirin® as compared to the ASPItest. In the ASPItest, a defined amount of arachidonic acid is used as the activator. However, in COLtest, platelets are activated through endogenous TXA2 and other TXA2 independent mechanisms.