Multiplate® analysis takes place in a single-use test cell which incorporates dual sensors and a teflon-coated stirring magnet. A key innovation of Multiplate® is the use of the Multiple Electrode Aggregometry (MEA) technology.
The principle of Multiplate® analysis is based on the fact that platelets become sticky upon activation and adhere and aggregate onto the metal sensor wires in the Multiplate® test cell. The test cell has a pipetting inlet and a cup portion with the sensor wires which extend into the blood sample. The sensor wires are made of highly conductive copper which are silver-coated. The test cell is connected to the instrument using a sensor cable and the electrical resistance between the sensor wires is recorded during the test. When activated, platelets adhere to the sensor wires and the electrical resistance between the wires increases.
- Whole blood, no sample processing
- Small sample volume (300 µl)
- Mini test cells use 175 µl of whole blood; smallest volume requirement for aggregometry testing. Ideal for pediatric platelet research or animal platelet studies.
- Up to 30 tests per hour
- Interactive electronic pipetting
- 5 independent channels for parallel testing
- Specific and sensitive reagents
- Standardized test procedures
- Open system for research applications
- Windows-based software
- New tests can be defined in the system software
- 1 × Multiplate® 5.0 Analyzer (MP0010)
- 1 × Multiplate® Electronic Pipette (MP0030)
- 1 × Multiplate® Pipette Tips (MP0040)
- 1 × Multiplate® Sensor Cables (MP0360)
- 1 × Multiplate® Test Cells (MP0027)
- 1 × Multiplate® Reagent Rack (MP0370)
- 1 × Multiplate® Keyboard (MP0441-EU)
- 1 × Multiplate® Mouse (MP0032)
- 1 × LCD monitor with hospital grade power cord (MP-MONITOR)
- 1 × Printer (PRINTER)
- 1 × Multiplate® Manual
The following reagents are available for Multiplate®
- Multiplate® ASPItest (08115826001)
- Multiplate® ADPtest (08115761001)
- Multiplate® COLtest (08115842001)
- Multiplate® RISTOtest (08116644001)
- Multiplate® TRAPtest (08116679001)
- Multiplate® Prostaglandin E1 (08115982001)
- Multiplate® GpIIbIIIa-Antagonist (08115869001)
The Multiplate® research analyzer records platelet aggregation at approximately 0.5 second intervals. The increase in impedance by the attachment of platelets onto the Multiplate® sensors is transformed to aggregation units (AU) and plotted against time.
Three parameters are calculated when performing research; Aggregation, AUC, and velocity. The most important parameter is the Area Under the aggregation Curve (AUC). AUC is recorded as Units or U. It is affected by the total height of the aggregation curve as well as by its slope and is best suited to express the overall platelet activity. The Aggregation (in AU) is the maximum height of the curve during the measurement period and the Velocity (in AU/min) is the maximum slope of the curve.
The output data calculated by the software is the mean value of the two independent sensors in the test cell. The correlation coefficient of the individual measurements is determined and the analysis is accepted when the correlation coefficient is greater than 0.98. Additionally, the difference of each curve from the mean curve (DIF) is calculated and is accepted when the difference is less than 20%.
[Note: An earlier version of the software expressed AUC as AU*min. The y-axis is aggregation in aggregation units (AU) and the x-axis is the time in minutes. To convert to current terminology, 10 AU*min = 1U.
Comparison of platelet aggregation using light transmission and multiple electrode aggregometry in Glanzmann thrombasthenia.
Awidi A, Maqablah A, Dweik M, Bsoul N, Abu-Khader A.
Platelets. Aug 2009; 20(5): 297-301.
Assessment of platelet function on whole blood by multiple electrode aggregometry in high-risk patients with coronary artery disease receiving antiplatelet therapy.
Paniccia R, Antonucci E, Maggini N, Romano E, Gori AM, Marcucci R, Prisco D, Abbate R.
Am J Clin Pathol. 2009; 131: 834-842.
Inhibition of platelet function with clopidogrel, as measured with a novel whole blood impedance aggregometer in horses.
Roscher KA, Failing K, Moritz A.
The Vet J. 2015; 203: 332-336.
Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood.
Tóth O, Calatzis A, Penz S, Losonczy H, Siess W.
Thromb Haemost. 2006; 96: 781-788.