The TECO®Urinary Collagen IV ELISA provides a method for the quantitative determination of collagen IV in human urine.
- Collagen IV Antibody coated Microassay Plate 12 strips of 8 wells (96 beak apart wells in total), in a frame. Ready to Use
- Collagen IV Calibrator—Standard A Ready to Use, 0.8 ug/L
- Collagen IV Calibrator—Standard B Ready to Use, 3.2 ug/L
- Collagen IV Calibrator—Standard C Ready to Use, 12.5 ug/L
- Collagen IV Calibrator—Standard D Ready to Use, 50 ug/L
- 10X Wash Buffer Concentrate
- Assay Buffer Ready to Use
- Enzyme Conjugate Anti-collagen IF mouse Fab’ conjugated to HRP. Ready to Use
- TMB Substrate Ready to Use
- Stop Solution 1M Sulphuric Acid, Ready to Use
- Plate Seal
The TECO® Urinary Collagen IV ELISA is designed for the assay of urinary collagen IV. It is a solid phase one-step sandwich ELISA. Collagen IV in the sample is bound simultaneously by a solid phase monoclonal antibody and a monoclonal antibody-enzyme conjugate directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme labelled antibodies. After removing unbound enzyme labelled antibody and sample, the plate is then incubated with enzyme substrate, resulting in the development of a color. The color developed is directly proportional to the amount of collagen IV in the sample.
Typical Calibration Curve
Typical calibration curve obtained using Serum Collagen IV ELISA. 4-Parameter plot of A450/630 nm versus ug/L Collagen IV. Assay range is 0.8-50 ug/L Collagen IV.
Collagen IV is a type of non-helical forming collagen found primarily is the basal lamina (basement membrane), and it is considered to constitute its basic framework. Urinary collagen IV levels are elevated in a variety of renal pathologies, particularly diabetic nephropathy. Urinary collagen IV is significantly higher in patients with non-insulin dependent diabetic mellitus (NIDDM) than in normal subjects and urinary collagen IV levels correlate with the deposition of collagen IV in the kidney. In diabetic subjects, urinary collagen IV is significantly increased in patients with microalbuminuria or overt proteinuria as compared to those with normoalbuminuria. Moreover, in diabetics with normoalbuminuria, those with elevated urinary collagen IV were at an increased risk for progression to microalbuminuria. Intensive therapy of diabetic nephropathy can slow the temporal increase in urinary collagen IV in diabetics indicating the potential of urinary collagen IV for studying the renal effects of new therapies. These results suggest that the measurement of Urinary Collagen IV might provide a useful biomarker for studying diabetic nephropathy.
In the field of renal transplantation, renal collagen IV levels are increased and increased urinary collagen IV levels are found in acute renal rejection, indicating the potential value of urinary collagen IV in studying these conditions.