The TECO® Serum Collagen IV ELISA provides a method for the quantitative determination of collagen IV in human serum.
- Collagen IV Antibody coated Microassay Plate 12 strips of 8 wells (96 break apart wells in total), in a frame. Ready to Use.
- Collagen IV Calibrator Stock Solution, 1000 ug/L
- 10X Wash Buffer Concentrate
- Dilution Buffer Ready to Use
- Enzyme Conjugate: Anti-collagen IV mouse Fab’ conjugated to HRP, Ready to Use
- TMB Substrate Ready to Use
- Stop Solution 1 M Sulphuric Acid, Ready to Use
- Uncoated Microassay Plate Ready to Use
The TECO® Serum Collagen IV ELISA is designed for the assay of serum collagen IV. It is a solid phase one-step sandwich ELISA. Collagen IV in the sample is bound simultaneously by a solid-phase monoclonal antibody and a monoclonal antibody-enzyme conjugate, each directed at different antigenic sites. This results in the collagen IV molecule being sandwiched between the solid phase and enzyme-labelled antibodies. After removing unbound enzyme-labelled antibody and sample, the plate is incubated with enzyme substrate. The resultant color development is directly proportional to the amount of collagen IV in the sample.
Typical Calibration Curve
Typical calibration curve obtained using Serum Collagen IV ELISA. 4-Parameter plot of A450/630 nm versus ug/L Collagen IV. Assay range is 15.6-1000 ug/L Collagen IV.
Collagen IV is a type of non-helical forming collagen found primarily is the basal lamina (basement membrane). Chronic liver disease comprises a number of progressive disorders which culminate in liver cirrhosis and which are characterized by excessive deposition of collagen.
Although various types of collagen (type I, III, IV, V and VI) increase in the liver with the progression of fibrosis, type IV collagen, a constituent of the basement membrane, is particularly noteworthy for the following reasons: its serum level correlates with hepatic levels of collagen IV, serum levels of collagen IV fall in response to effective therapy, and it is the earliest type of collagen to be synthesized during experimental liver injury. Serum collagen IV levels are elevated in a variety of liver diseases, and have been found to be predictive of therapy response in Hepatitis C infection and to be sensitive indicators of therapy response in abstaining alcoholics.