Thrombodynamics® PLS is a reagent and consumable kit used to perform measurements of spatiotemporal dynamics of fibrin clot formation and thrombin generation in blood plasma samples on the T2T Analyzer.

Thrombodynamcis PLS Assay Kit

  • Reagent I. 10 Orange Plastic Tubes:
    Contains a lyophilized mix of a contact pathway activation inhibitor, fluorogenic substrate for thrombin activity measurement, and additives.
    Reagent I is ready to use as packaged
  • Reagent II. 10 Blue Plastic Tubes:
    Contains a lyophilized mix of calcium salt and additives
    Reagent II is ready to use as packaged
  • Reagent PLS. 1 dark or neutral vial:
    Contains a lyophilized suspension of phospholipid vesicles
    Reagent PLS should be reconstituted with 300 µL of distilled water prior to use
  • Measurement Cuvette. 5 Individually wrapped cuvettes:
    A plastic vial with 2 thin channels for plasma samples
    Measurement cuvettes are ready to use as packaged
  • Activating Insert. 5 Individually wrapped inserts
    A plastic comb with immobilized tissue factor in its bottom end faces
    Activation insert is ready to use as packaged

In the Thrombodynamics® test kits, protein tissue factor (TF) located on the end face of the activating insert mimics the physiology of a damaged vasculature or inflammation.  A basal level of active serine protease Factor VIIa binds this TF to become a fully functional protease that eventually leads to fibrin polymerization and clot formation.  The on board dark-field camera images this fibrin polymerization over time allowing for quantification of multiple parameters of blood coagulation using special computerized mathematical techniques.

Additionally, the PLS kit contains phospholipids and fluorogenic substrate.  The on board fluorescence detecting camera images this thrombin generation over allowing for the quantification of multiple parameters of thrombin generation in both time and space using special computerized mathematical techniques.