Technochrom® FVIII:C is a reagent kit for the photometric determination of coagulation factor VIII activity in plasma and factor VIII concentrates by measuring factor Xa generation with a chromogenic substrate. The kit shows an excellent correlation with one and two stage Factor VIII assays and a linear calibration curve between 0 and 130%. It contains all components, is insensitive to heparin up to 10 IU/ml and is suitable for plasma and concentrates. It can be used for assaying Factor VIII deficiencies.

For the use with Ceveron® alpha.

  • Substrate: 2 x 2, FXa-1+ αNAPAP 10 µmol / vial F Xa-1, 0.012 µmol / vial αNAPAP
  • Reagent A: 2 x 2, Phospholipid, albumin
  • Reagent B: 2 x 2, F IXaß, F X, Ca++, albumin, thrombin
  • Ref. Stand. FVIII 1: 1 x 1, ~ 130 % or 1.30 IU FVIII / ml
  • Ref. Stand. FVIII 2: 1 x 1 , ~ 70 % or 0.70 IU FVIII / ml
  • Ref. Stand. FVIII 3: 1 x 1, ~ 10 % or 0.1 IU FVIII / ml
  • Ref. Stand. FVIII 4: 1 x 1, < 0.5 % or 0.005 IU FVIII / ml
  • F VIII dilution buffer: 2 x 25, 3.4 g/l Imidazole, 5.85 g/l NaCI, 0.2% Albumin, pH 7.4
  • F VIII reaction buffer: 2 x 8, 6.06 g/l Tris, 3.03 g/l Na2ETDA, 25 g/l NaCI, pH 8.3

MATERIAL REQUIRED (not supplied with the kit)

  • Pipettes
  • Distilled water
  • Control Plasma Normal and Abnormal
    Coagulation Control N for Ceveron® 5 x 1 ml, Catalog# 5020020
    Coagulation Control N for Ceveron® 50 x 1 ml, Catalog# 5020025
    Coagulation Control A for Ceveron® 5 x 1 ml, Catalog# 5021035
    Coagulation Control A for Ceveron® 50 x 1 ml, Catalog# 5021040
F IIa
FVIIIsample + (FIX-PL- Ca++)FVIIIa-FIX-PL-Ca++-complex
FVIIIa-FIX-PL-Ca++-complex
FXFXa
Substrate-FXa-1Substrate-FXa-1 + pNA