ABP TRAP-6 is a synthetic hexapeptide (SFLLRN-NH2) that mimics the action of thrombin. It is used for platelet aggregation and functional studies for the evaluation of normal platelet function on the Multiplate® Analyzer and Stasys® Analyzer and for the research evaluation of qualitative platelet function inhibition. It is sensitive to the action of glycoprotein (Gp) IIb/IIIa receptor antagonists and/or a deficiency of GpIIb/IIIa receptors. As TRAP-6 activates the PAR-1 and PAR-4 receptors, it is insensitive to both P2Y12 inhibitors and aspirin.
ABP TRAP-6 is provided as a single vial containing 5.0 mg of TRAP-6 amide powder. The molecular weight is 747.90. TRAP-6 may be transported at ambient temperature but should be stored at -20oC once received. Following reconstitution with physiologic saline, TRAP-6 may be stored for an extended time at -80oC. Prior to use, frozen TRAP-6 should be warmed to RT and kept at 2oC-8oC when not in use.
In the TRAPtest, platelets are activated by TRAP-6, a peptide that mimics the activation of platelets by the action of thrombin. Thrombin is the most potent platelet activator. The action of thrombin is not blocked by the antiplatelet agents Aspirin® or clopidogrel. However, thrombin (and TRAP-6) activated platelets are blocked by the action of GPIIb/IIIa antagonists (e.g., ReoPro®, Aggrastat® and Integrilin®).