Ceveron® TGA RC Low Kit (thrombin generation assay reagent C Low) is a reagent kit for use on the Ceveron® Alpha, Ceveron® t100, or Ceveron® s100 instruments. The TGA RC Low trigger reagent is most useful for measurement of
- Hypercoaguable plasma
- Changes in the positive feedback loop of the intrinsic pathway activation
- Researching thrombogeneity of microparticles
Ceveron® TGA RC Low is based on monitoring the fluorescence generated by the cleavage of a fluorogenic substrate by thrombin over time, upon activation of the coagulation cascade in plasma by Ceveron® TGA RC Low trigger.
The trigger is composed of tissue factor and negatively charged phospholipids specially adapted to detect very sensitive changes in the positive feedback loop of intrinsic pathway activation following its activation by the extrinsic pathway. This gives the assay high sensitivity to thrombophilic tendencies.
From the changes in fluorescence over time, the concentration of thrombin (nM) in the sample can be calculated using the respective thrombin calibration curve. The increase in thrombin concentration over time allows the calculation of the thrombin generation curve and to calculate various thrombin generation parameters.
|2x 1mL||Ceveron® TGA RC Low||Trigger reagent with low concentration of phospholipid micelles containing rhTF in Tris-HEPES-NaCl buffer. Lyophilized|
|2x 1.5mL||Ceveron® TGA BUF||Tris-HEPES-NaCl buffer. Lyophilized|
|2x 3mL||Ceveron® TGA Sub||1 mM fluorogenic substrate Z-G-G-R-AMC. Lyophilized|
|2x 1mL||Ceveron® CONT H||Human plasma with increased thrombin generation. Lyophilized|
|2x 1mL||Ceveron® CONT L||Human plasma with decreased thrombin generation. Lyophilized|
|1x 25mL||Calcium Chloride solution, 25 mM||CaCl2, 25 mM. Ready-to-Use|
Stability and Storage
The expiration date printed on the labels is applicable so long as the vials remain unopened. Stability following reconstitution
|Reagent||Ceveron® Cooling Block||2o-8oC|
|Ceveron® TGA RC Low||8 Hours||5 Days|
|Ceveron® TGA BUF||8 Hours||5 Days|
|Ceveron® TGA Sub||8 Hours||5 Days|
|Ceveron® CONT H||4 Hours||–|
|Ceveron® CONT L||4 Hours||–|
|Calcium Chloride solution, 25 mM||7 Days||4 Weeks|
This example normal range was determined using 100 heathy, normal donor PPP sample. Each laboratory should establish and control its normal range.
|Reagent||nM Peak Thrombin||nM AUC|
|Ceveron® TGA RC Low Kit||43-368||1236-2945|
Abdominal and thoraco-abdominal aortic aneurysm (AAA/TAA)
Subjects with abdominal and thoraco-abdominal aortic aneurysm (AAA/TAA) show abnormal high peak thrombin, which correlates with aneurysm size.
Preeclampsia and activation of thrombin generation in the third trimester
During the manifestation of the disease, subjects with preeclampsia have significantly higher thrombin generation compared to patients with normal pregnancies.