The BioTek® FLx800™ TBI Fluorometer is a multi-detection microplate reader that combines excellent specifications and performance with convenience and ease of use. This design incorporates powerful performance at a price much lower than traditional fluorescence-luminescence microplate readers. The FLx800™ line includes several models with options that meet the specific needs of research and OEM users. Top and bottom reading are available to read anything from 6-well to 384-well microplates, and PCR plates. An optional external dispenser module allows to run applications such as ion channel assays and flash luminescence assays.

For assaying thrombin generation using Technothrombin® TGA you need a fluorescence reader equipped with filters 360 nm and 460 nm (excitation/emission) as the BioTek® FLx800™ TBI with an adapted software for Technothrombin® TGA.

  • Custom-mapped fiber optics for optimal signal transfer
  • Selectable, automated top- or bottom-probe reading for best sensitivity
  • Typical fluorescence sensitivity to 5 pg sodium fluorescein
  • Glow luminescence reading with top detection for best sensitivity
  • Incubation to 50ºC for temperature-sensitive assays
  • PCR tube-reading option available
  • Reads 6- to 384-well formats and PCR plates
  • Convenient front panel accessory access makes changing filters simple
  • Powerful on-board data reduction software
  • Computer control
Detection modesFluorescence intensity
Read methodsEndpoint, kinetic, well area scanning (under computer control)
Microplate types6- to 384-well plates
Temperature control4 °C above ambient to 50 °C (I models)
ShakingLinear (I models)
SoftwareGen5™ Data Analysis Software
Gen5 Secure for 21 CFR Part 11 compliance (option)
Fluorescence Intensity
Light sourceTungsten halogen
Wavelength selectionFilters
Wavelength range300 – 700 nm (850 nm option)
Dynamic range5 decades
SensitivityFluorescein 5 pM (1 fmol/well 96-well plate)
Wavelength range300 – 700 nm
Dynamic range5 decades
Sensitivity100 amol ATP (flash)
Reagent Dispensers
Supported detection modesFluorescence
Number1 syringe pump
Dead volume1.1 ml, 100 µl
Dispense volume5 – 1000 µl in 1 nm increments
Physical Characteristics
Power100 – 240 Volts AC. 50/60 Hz.
Dimensions15″W x 16″D x 9″H
(38.1 x 40.64 x 22.89 cm)
Weight30 lbs (13.6 kg)

Specifications are subject to change. Performance values represent the average observed factory test values.


Bio-tek FLx800 Fluorescence Microplate ReaderBioTek’s Gen5™ software offers a logical interface designed to easily flow from reading parameters, to plate layout, to powerful data reduction, and finally to flexible data output options. The results of this intuitive design, including the exclusive StepWise™ protocol and data reduction tools and the power of Gen5’s data reduction, will be evident in increased laboratory efficiency.