Growth differentiation factor-15 (GDF-15) is a stress responsive cytokine. It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition.

GDF-15 is a biomarker for many disease states.  The expression of GDF-15 is dramatically increased in many types of cancer. Tumor overproduction of GDF-15 induces appetite suppression and subsequent weight loss. Overexpression or pharmacological infusion of GDF15 reduces food intake and body weight and improves metabolic profiles in various metabolic disease models in both rodents and monkeys.  Furthermore, in biopsy-proven NAFLD human subjects, findings suggest that GDF15 may serve as a novel biomarker of advanced fibrosis.

 

 

 

REAGENTS SUPPLIED

Each kit is sufficient for one 96-well plate and contains the following components:

  • Microtiter Strips (96 wells), coated with a monoclonal antibody against human GDF-15, sealed
  • 10×Wash buffer, 50 mL
  • 5×Assay buffer, 20 mL
  • 100×Detection antibody solution, a biotin labelled polyclonal antibody against human GDF-15, 0.12 mL
  • Human GDF-15 standard, 500 pg of recombinant human GDF-15, lyophilized
  • 200×STP-HRP solution, 0.06 mL
  • Substrate solution, 12 mL, ready for use
  • Stop solution, 12 mL, ready for use

 

OTHER MATERIALS REQUIRED, BUT NOT PROVIDED

  • Pipettes and pipette tips
  • 96-well plate or manual strip washer
  • Buffer and reagent reservoirs
  • Paper towels or absorbent paper
  • Plate reader capable of reading absorbency at 450 nm

Sample type: serum, plasma and cell culture supernatant samples

Sample volume:  100 uL, Serum or plasma sample generally requires a 10-fold

Species:  Human

Detection Range:  7.8-500 pg/mL

Sensitivity:  7.8 pg/mL

Run time:  < 3 hours

Storage:  2-8°C upon receipt, and all reagents should be equilibrated to room temperature before use