Technoview® LMW Calibrator Set is a set of plasma-derived calibration samples with low molecular weight heparin (LMWH) concentrations ranging from ~0-1.6 IU/ml. The set can be used for establishing a standard curve in research measurements of low molecular weight heparin. It is optimized for use with anti-Xa chromogenic assays and is frequently used in conjunction with the Technoview® LMW heparin low, medium and high controls in the Technochrom® anti-Xa chromogenic assay.
- Allows for establishment of a calibration curve covering a range of 0-1.6 IU/ml of low molecular weight heparin
- Optimized for use with anti-Xa (without exogeneous antithrombin) research assays
5 x 1 ml vials with a range of low molecular heparin (LMWH) concentrations:
- Cal 1: 0 IU/ml
- Cal 2: 0.4 IU/ml
- Cal 3: 0.8 IU/ml
- Cal 4: 1.2 IU/ml
- Cal 5: 1.6 IU/ml
Calibrators are lyophilized and prepared from pooled human citrated plasma supplemented with different concentrations of low molecular weight heparin. The plasma contains stabilizers but no bactericide additives. They are calibrated against the International Standard preparation for LMWH, 01/608.
Heparin is a widely used antithrombotic agent that can be administered via an intravenous or subcutaneous route. It is naturally occurring and typically derived from bovine or porcine tissue. The anticoagulant effect of this sulfated glycosaminoglycan results from its binding to antithrombin (AT), the major inhibitor of the coagulation cascade. Binding induces a conformational change in the AT molecule, which greatly accelerates the AT inhibition of several coagulation proteases. The accelerated inhibition of thrombin and Factor Xa by heparin is the primary basis for its anticoagulation properties.
Two forms of heparin are used therapeutically: unfractionated heparin (UFH), with an average molecular weight (MW) of 15,000 and low molecular weight heparin (LMWH), with a MW between 4,000 and 6,500. The lower MW results in LMWH acting primarily on Factor Xa, whereas UFH efficiently inhibits both thrombin and Factor Xa. Both heparin forms should be monitored. UFH can be measured with an aPTT or an anti-Xa chromogenic assay while LMWH should be measured using and anti-Xa chromogenic assay. Note that this calibrator set is for research use only in the US and Canada.