Technoclone Anti-Fibronectin Monoclonal Antibody 2FN

IgG; binds to human fibronectin. Applications include ELISA and Western Blot.




Affinity chromatography on Protein A sepharose


Human fibronectin, purified from plasma

Generation of hybridoma cells

NSO myeloma cell line X spleen cells BALB/c mice


2 FN binds to human plasma fibronectin

Suggested working concentration

10 µg/ml.

Lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline (PBS) pH 7.4, containing 20 mg/ml mannitol

Reconstitute with 0,5 mL distilled water for preparing high dilutions use a protein containing diluent (e.g. 1% BSA in PBS)

Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.