Technoclone Anti-alpha2-Antiplasmin 3AP
IgG1; reaction with native alpha-2-antiplasmin and with the MW = 8,000 peptide derived by SDS gel electrophoresis of plasmin-alpha-2-antiplasmin complexes as demonstrated by immunoblotting. Half maximal binding to coated alpha-2-antplasmin at 8 ng/ml. The antibody can be used for detection of uncleaved α-2-antiplasmin and separation of the plasminogen binding form of α-2-antiplasmin from the non-plasminogen binding form.
Binds native α-2-antiplasmin and the Mr = 8000 peptide derived by SDS-gel-electrohporesis of plasmin-α-2-antiplasmin complex (1). Half maximal binding to coated α-2-antiplasmin at 8 ng/ml.
Hybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified α-2-antiplasmin (1).
The antibody is purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.
The antibody is lyophilized from a 1 mg/ml solution in isotonic phosphate buffered saline, pH 7.4, containing 0.02% sodium azide and 20 mg/ml mannitol. It is supplied in vials of 500 µg and should be reconstituted with 0.5 mL distilled water. For extensive dilutions a protein containing solution should be used (e.g. 1% bovine serum albumin in PBS). Lyophilized antibody should be stored at 4°C. Reconstituted antibody should be aliquoted and stored at -20°C or lower. Avoid repeated freeze-thaw cycles.
1) E. Hattey, J.Wojta, and B.R.Binder: Monoclonal antibodies against plasminogen and α-2-antiplasmin: Binding to native and modified antigens. Thrombosis Research 45: 485, 1987