Technochrom® Factor VIII:C is a reagent kit for the photometric determination of coagulation factor VIII activity in plasma and factor VIII concentrates by measuring factor Xa generation with a chromogenic substrate. The kit shows an excellent correlation with one and two stage Factor VIII assays and a linear calibration curve between 0 and 130%. It contains all components, is insensitive to heparin up to 10 IU/ml and is suitable for plasma and concentrates. The Technochrom® Factor VIII:C kit can be used for assaying Factor VIII deficiencies as well as studying Factor VIII substitution.

  • 2 x 2 Substrate FXa-1+ αNAPAP, 10 µmoL / vial F Xa-1, 0.012 µmoL / vial αNAPAP
  • 2 x 2 Reagent A, Phospholipid, albumin
  • 2 x 2 Reagent B, F IXaß, F X, Ca++, albumin, thrombin
  • 1 x 1 Ref. Stand. FVIII 1, ~ 130 % or 1.30 IU FVIII / ml
  • 1 x 1 Ref. Stand. FVIII 2, ~ 70 % or 0.70 IU FVIII / ml
  • 1 x 1 Ref. Stand. FVIII 3, ~ 10 % or 0.1 IU FVIII / ml
  • 1 x 1 Ref. Stand. FVIII 4, < 0.5 % or 0.005 IU FVIII / ml
  • 3 x 30 FVIII dilution buffer, 3.4 g/L Imidazole, 5.85 g/L NaCI, 0.2% Albumin, pH 7.4
  • 2 x 8 FVIII reaction buffer, 6.06 g/L Tris, 3.03 g/L Na2ETDA, 25 g/L NaCI, pH 8.3

MATERIAL REQUIRED (not supplied with the kit)

  • Pipettes – Distilled water
  • For the endpoint method: 20 % acetic acid.
  • Control Plasma Normal and Abnormal:
    Coagulation Control N, 5 x 1 ml, Catalog # 5020040
    Coagulation Control A, 5 x 1 ml, Catalog # 5021055
FIIa
FVIIIsample + (FIX-PL- Ca++)FVIIIa-FIX-PL-Ca++-complex
FVIIIa-FIX-PL-Ca++-complex
FXFXa
Substrate-FXa-1Substrate-FXa-1 + pNA