A PKA standard for use in the determination of Prekallikrein Activator (PKA) in Human Blood Products and Biologicals according to the European and US Pharmacopoeias. This PKA standard is ideal to use with in-house developed PKA assays to increase convenience and streamline workflow.
The PKA Assay Standard is calibrated against the 3rd International Standard for PKA and is intended for use in assays of PKA based on the methods outlined in the European and US Pharmacopoeias.
In the Pharmacopoeia method for PKA assays, a Human PreKallikrein pool is first activated to plasma kallikrein by PreKallikrein activator, (PKA -FXIIa) in the test sample. The kallikrein formed then releases p-nitroaniline (pNA) from a chromogenic kallikrein substrate. The amount of pNA released is measured photometrically at 405 nm and is proportional to the level of PKA present in the preparation.
PKA Standard ~32 IU/ml (5x 0.5ml)
Reconstitute in 0.5 ml of distilled water, leave to stand at room temperature for 10 minutes and mix by gentle swirling. This gives a batch specific PKA concentration of approximately 32IU/ml.
The standards should be stored at 2-8oC until the batch specific expiry date indicated on the box label.
Once reconstituted, the standard can be stored for up to 8 hours at 4ºC before use, or at -20ºC for 6 months.
Additional reagents required:
- Chromogenic Kallikrein Substrate
H-D-Pro-Phe-Arg-pNA (S820340 Chromogenix S-2302™)
- Human Prekallikrein Pool
(PW30304 PKK Pool, 4 x 2.0 ml, is recommended)
- PKA Assay buffer