A Human Prekallikrein Pool for the determination of Prekallikrein Activator (PKA) in Human Blood Products and Biologicals according to the European Pharmacopoeia.
The Human Prekallikrein pool is prepared according to the procedure recommended by the European Pharmacopoeia (EP) for assays of Prekallikrein Activator (PKA). The product is intended for use in assays of PKA based on the EP method.
In the EP method for PKA the Human PreKallikrein pool is first activated to plasma kallikrein by PreKallikrein activator (PKA -FXIIa) in the test sample. The kallikrein formed then releases p-nitroaniline (pNA) from a chromogenic kallikrein substrate. The rate at which the pNA is released is measured photometrically at 405 nm in a microtitre plate reader and the amount of pNA released is proportional to the amount of PKA present in the preparation.
- Human PreKallikrein (2x 2.0ml)
- Buffer A Concentrate (1x 6ml)
The reagent pack should be stored at 2-8°C before use.
Additional Material Required
- Chromogenic Kallikrein Substrate: H-D-Pro-Phe-Arg-pNA.
- PKA Standard